timsTOF fleX with ESI/MALDI capability for SpatialOMx at ASMS 2019 launched by Bruker

Bruker has announced highly innovative new mass spectrometry products and workflows:

1.   SpatialOMx™ Life Sciences and Translational Mass Spectrometry Imaging
2.   4D Proteomics and 4D Phenomics Innovations

At the 67th American Society for Mass Spectrometry Conference (ASMS) being held June 2-6 in Atlanta, Bruker is announcing highly innovative new mass spectrometry products and workflows. Bruker introduces the novel timsTOF fleX™ mass spectrometer, which includes a software-switchable MALDI source adapted to the ESI timsTOF Pro™ platform.  This new, combined ESI/MALDI capability enables spatially-resolved omics, SpatialOMx™, on a single instrument.

The timsTOF fleX comes with Bruker's proprietary 10kHz SmartBeam™ 3D laser with true pixel fidelity for rapid, label-free MALDI imaging at high-spatial resolution, while fully preserving the unparalleled 4D proteomics and phenomics sensitivity of the timsTOF Pro in ESI mode.

With this unique SpatialOMx approach, researchers gain insights into spatial molecular distributions in tissues from MALDI imaging, to guide 4D omics molecular expression studies on proteins, low-level cancer antigen peptides, lipids, glycans, metabolites, or xenobiotics, which cannot be observed by traditional staining or labeling techniques.

MALDI-guided SpatialOMx allows for specific targeting of cell sub-populations for subsequent ESI-TIMS/PASEF-based dda or dia 4D proteomics or 4D lipidomics/metabolomics. Both can now be performed on a single robust instrument, the timsTOF fleX, with the ultra-high sensitivity needed to advance single-cell biology research, as a perfect complement to single-cell transcriptomics by RNA-seq.

The robust, ultra-high sensitivity timsTOF fleX bridges the divide between molecular tissue imaging and body fluids analysis by offering nanoLC-TIMS-MS/MS on the same instrument. This unique combination will make the timsTOF fleX an invaluable research tool for spatially-resolved 4D proteomics and phenomics, as well as for advancing single-cell biology, pharmaco-proteomics, and translational 4D cross-omics workflows on large cell numbers or patient cohorts."

Bruker is also launching IntelliSlides™ specifically designed to automate timsTOF fleX workflows.  The IntelliSlidescome pre-inscribed with software-readable teach marks on the conductive surface to indicate where to place the tissue sample, a bar code, and a tracking number.

IntelliSlides automation removes sources of inefficiency from sample loading, as they are inherently correctly labeled, oriented properly, and MALDI image registration occurs at the touch of a button.  With specialized software, IntelliSlides now make MALDI Imaging even easier for everyone.

Moreover, Bruker introduces SCiLS Lab 2020 MALDI imaging software, now integrated with MetaboScape5.0 for providing automated annotations of lipids and metabolites in tissue molecular images in spatialOMx.  This unique combination automatically matches ions measured on a tissue to molecular information in metabolomics and lipidomics workflows, highlighting biologically relevant pathway information using MALDI imaging.

The new integrated imaging and metabolomics workflow also support data from Bruker's scimaX™ MRMS platform, as well as from the new timsTOF fleX. MetaboScape's unique T-ReX 2D algorithm performs feature extraction, de-isotoping, and ion deconvolution on MALDI imaging datasets.

Within MetaboScape, molecular features are annotated based on accurate mass and isotopic fidelity using SmartFormula™ and molecular information, e.g. from public databases such as HMDB and LipidMaps. MetaboScape now also offers the unique ability to increase ID scoring confidence by integrating accurate TIMS collision cross-sections (CCS) from timsTOF analyses.

Identifications flow back to SCiLS Lab for fully annotated molecular images. SCiLS Lab is the market-leading software for MS imaging, while MetaboScape is the software solution of choice for the identification of metabolite markers and pathway mapping.

DiaPASEF and MBR-ddaPASEF Advances in 4D Proteomics

Bruker's revolutionary timsTOF™ Pro for next-generation nLC-TIMS-MS/MS 4D proteomics has been further enhanced by combining PASEF with data-independent (DIA) acquisition, in diaPASEF for ultra-high sensitivity bottom-up proteomics.

While the unmatched duty-cycle of data-dependent acquisition (DDA) PASEF dramatically improved sensitivity and depth of coverage using short nanoLC runtimes, the stochastic nature of DDA results in missing values, an issue significantly improved by diaPASEF workflows, or by match-between-runs (MBR) for ddaPASEF.

The novel diaPASEF1 workflow, shown at ASMS 2019 as work-in-progress available to methods development customers, uses overlapping windows in the ion mobility domain to trigger MS/MS, efficiently using the quadrupole to transmit the precursor ions at high sensitivity.

By using the inherent duty-cycle advantage of PASEF, the new diaPASEF workflow typically results in a 30% improvement, now with over 7,000 proteins identified in a 120-minute single-shot experiment with 200 ng of HeLa digest injected.  Data analysis, including 4D feature alignment in mass, retention time, ion mobility, and intensity, is performed using the new Mobi-DIK software, which is based on the OpenMS software developed in the group of Professor Hannes Roest at the University of Toronto.

The inStageTip (iST) technology removes detergents, polymers, salts, lipids, and other contaminants, and has exceptional peptide recovery.  New iST protocols enable robust and reproducible sample preparation with a significant time advantage (over 40 hrs saved) compared to common protocols, and iST is suitable for integration in semi-automated, high-throughput sample prep.

For proteomics software, Bruker and Genedata announced a partnership for the Genedata Expressionist software platform, which now supports the timsTOF Pro 4D omics format. Native support of the latest timsTOF Pro data file format opens new opportunities for streamlining and automating the most complex proteomics, metabolomics, and biotherapeutics characterization studies.

The power of intelligent precursor selection for deep proteome coverage with fast gradients of the timsTOF Pro in combination with the scalability and speed of Genedata Expressionist enables the implementation of a next-generation host cell protein (HCP) analysis platform.

Novel Ultra-high Sensitivity 4D Lipidomics Workflow

Building on the success of MetaboScape® and CCSPredict™, Bruker announces advances to ultra-high sensitivity 4D lipidomics workflows on the timsTOF Pro and timsTOF fleX platforms, demonstrating high-throughput and high-sensitivity measurements using LC-TIMS-MS/MS.

Optimization of novel PASEF 4D lipidomics methods now enables the number of identified lipids in single-shot analysis to be almost doubled, whilst obtaining attomole sensitivity.  This innovative workflow uses nanoLC-TIMS-PASEF to quantify approximately 500 lipids with very high quantitative accuracy and reproducibility from just a few thousand cells, in addition to building a library of more than 1,000 accurate CCS values from human plasma, mouse liver, and human cancer cells.

Post a Comment